Fiji imagej user guide7/3/2023 ![]() ![]() – Optimized parameters folder: tables containing optimized parameters will be stored here if optimization is performed. ![]() – ROIs folder: ROIs will be stored in this folder. – Non-processed images folder: images that will not be used for the analysis will be relocated into this folder. We suggest creating subfolders to allocate images from similar experimental groups. This is the way this macro has to allow continuing with the work after interruptions. – Processed images folder: images will be moved to this folder after ROI design. It is recommendable to create subfolders where images from similar experimental groups are stored. Preparing images for analysis Before playing around with the macro it is recommended to create the following folders: – Original images folder: this folder should contain the images to be analyzed. To solve the issue of malfunction due to FIJI updates we have also included the version of FIJI used when developing FLEYE plugin in FIJI_with_FLEYE.zip files (available for Win32 and Win64 for MacOs we provide a FIJI version that does not include the FLEYE plugin). To install the package unzip the FLEYE.zip file into the plugins folder of FIJI. FLEYE Installation The package includes three ImageJ macros that will allow the user to select in each image the regions of interest (ROI) for analysis, optimize parameters and analyze the images. ![]() Copyright (C) 2014 Cristina Rueda Sabater, Sergio Díez Hermano, Diego Sánchez Romero, María Dolores Ganfornina Álvarez and Jorge Valero Gómez-Lobo ( ). FLEYE user guide FLEYE USER GUIDE FLEYE is a package of ImageJ macros developed to classify fly eyes attending to the distribution of their ommatidia, using images of the eye surface as starting data. ![]()
0 Comments
Leave a Reply. |